Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRDLREIGLTKVKKND---MVYYVLVNETEKIDLVEFLSHHLEGVAR--AEFTL---------VLHTKLGEASVLANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK 3LAP Chain:A ((18-156)) --RAGRQARIVAILSSAQVRSQNELAALLAAEGIEVTQATLSRDLEELGAVKLRGADGGTGIYVV----PE------GVSGGTDRMARLLGELLVSTDDSGNLAVLRTPPGAAHYLASAIDRAALPQVVGTIAGDDTILVVAREP-----------------

General information: TITO was launched using: RESULT: Template: 3LAP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 402 -16477 -40.99 -138.46 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -40.99 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_3LAP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LAP-query.scw PDB file : Tito_Scwrl_3LAP.pdb: