Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQQRNILATLGIDVWIPRTQVCQKNNAHTLWRDQVVEPHESITVPTIDVPAFEQKNTQPQVFEIPKVVEEPPIVVAEVSQPEILVEKPKVIEQETITPFELQAYCLEKCVIFVDVTALETEEKQLWANIQKAKVGRYSEL------RWPFPLAAYQDQRGVGSYIQGFLDAV---AAEKKILCLGKCAYI---------------------QHANIIHLASLKEMLDKPL--LKKRLWQLMQDNNE 1L9G Chain:A ((62-184)) --------------------------------------------------------------------------------------------------------------------------RAGMLLTELLRESGIRREDVYICNVVKCRP-PNNRTPTPEEQAACGHFLLAQIEIINPDVIVALGATALSFFVDGKKVSITKVRGNPIDWLGGKKVIPTFHPSYLLRNRSNELRRIVLEDIEKA--

General information: TITO was launched using: RESULT: Template: 1L9G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 215 -785 -3.65 -8.62 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -3.65 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_1L9G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L9G-query.scw PDB file : Tito_Scwrl_1L9G.pdb: