Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIHYPVYFFFELIQASCNEYIASGSNKEDIMLLNRK-----HSLVSCFDLELLETHPVKIKNSKVKQKNFITVSLEFSFKKIPPIF 3ISQ Chain:A ((339-377)) --------LFLEVIQRH--NH--QGFGAGNFNSLFKAFEEEQNLRGNLTNM------------------------------------

General information: TITO was launched using: RESULT: Template: 3ISQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 18 -1691 -93.94 -49.74 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -93.94 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.080

(partial model without unconserved sides chains): PDB file : Tito_3ISQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ISQ-query.scw PDB file : Tito_Scwrl_3ISQ.pdb: