Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMLTSKASLRLTLLASAIFLVACQPKSDPKESEDQQKPAVVEQKPVELTLKGETVPSKVTLPDCDGKTCPEFTVARLQ-SNFPFIDKIIDQQVLK-ALGQILEIAEPDAKAAQADKKTEASTAAATEQQDSFDAQVQRYANSFIDLDNDLKALSSNHQINLLVKPKIIQSQGKVVTVVVNSSSYLGGAHG-SAAQQYYNFDLKKEKQVKLEG-LLRPEKKAALEKLAHEAFKAWVTDSKLVNSVSEYEQAWPFKLTENFLLGDQGLILQYGEYEIGPYVVGLPRLVIPYDQLQDVLKEEYLPQPKAKPASTPAVKSTS 4E72 Chain:A ((26-247)) --------------------------------------------GLDDPLPTERLASEHLKPGCQGEQCPLVNIDTLKFPDEPQLDPIVERALLEMTRTPLPASLAAYERQFLDS---------------------------------------AEPGWSSYLQAKVREQHDGLVIIELSSYLFTGGAHGMP-GRGFINYDRRQHKVLSLQDMLV-PGQEEAFWKQAELAHKAWLLANKL-DQDADFQKTWPFQRTPHVALTFGAVTLKYDAYSIAPYSYAHPELKIPYPRLNGIVKPNLFPGR--------------

General information: TITO was launched using: RESULT: Template: 4E72.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1 19 18.50 0.09 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 18.50 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : ?

(partial model without unconserved sides chains): PDB file : Tito_4E72.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E72-query.scw PDB file : Tito_Scwrl_4E72.pdb: