Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYFMSFKDELAAQVAQRRTFAIISHPDAGKTTMTEKLLLWGKAIQVAGMVKSRKSDRAATSDWMEMEKERGISITTSVMQFPYKGHTINLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVEERTIKLMEVCRMRDTPIISFVNKMDREIREPLELLDEIENVLNIRCVPITWPLGMGRDFAGVYNILEDKLYVYKAGFGSTITDIEVRDGY-DHA-DIREKVGELAWASFEESLELVQMANEPLDRGLFLQG--------KQTPVLFGTALGNFGVDHVLDAFMHWAPEP------KAHPTQERVVEAKEE---GFSGFVFKIQANMDPKHRDRIAFMRICSGKYEKGLKMNHVRISKEVRISDALTFLAGEREHLEEAWPGD---IIGLHNHGTIQIGDTFTSGENLHFTGIPHFAPEMFRRVRLKDPLKSKQ--LQKGLKELSEEGAT-QVFMPQISNDLIVGAVGVLQFDVVAYRLKEEYKVDCVYEPVSVNTVRWIHCDDEKILNEFKKKAHDQLSIDGGGHLTYLAPSRVNLQIMQERWPDIEFRSTREH 4M1K Chain:A ((13-474)) -----------------RNIGIAAHIDAGKTTTTERILYYTGRI-------------------------------AAVTTCFWKDHRINIIDTPGHVDFTIEVERSMRVLDGAIVVFDSSQGVEPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTMQERLGARPVVMQLPIGREDTFSGIIDVLRMKAYTYGNDLGTDIREIPIPEEYLDQAREYHEKLVEVA-ADFDENIMLKYLEGEEPTEEELVAAIRKGTIDLKITPVFLGSALKNKGVQLLLDAVVDYLPSPLDIPPIKGTTPEGEVVEIHPDPNGPLAALAFKIMA--DP-YVGRLTFIRVYSGTLTSGSYVYNTTKGRKERVARLLRMHANHREEVEELKAGDLGAVVGLKETIT---GDTLVGEDAPRVILESIEVPEPVIDVAIEPKTKADQEKLSQALARLAEEDPTFRVSTHPETGQTIISGMGELHLEIIVDRLKREFKVDA--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4M1K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2190 -50076 -22.87 -122.14 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -22.87 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_4M1K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M1K-query.scw PDB file : Tito_Scwrl_4M1K.pdb: