Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSYANLLKPLHLGFTTIKNRVVMGSMHTGLEDRFFNYPKLAAYFEE-RAKGGVGLIVTGGISPNRQGWLAP-AGGTMNSLFDIPQHRLVTHAVHKHGSKILMQILHAGRYG------YQPFVVSSSPIK-SPISPFKPRQLSEKNILSTIEDYAQCADIAKKAGYDGVEIMGSEGYL-INQFLSRHVNQRTDRWGGEIENRMRFPVEIVKAIRAKVGEKFIICFRLSLLDLVHDGNTMQEVVTVAKALEKAGVTLLNTGIGWHEARVPTIVTS--VPRAAFVDYTAHVKQHISIPVIASNRINMPETAEEILATGKADMIQMARPLLADAFWVNKTATNRVDEINTCIACNQACLDHAFKNKRVSCLVNPRAGHETELVY----LKTKQPK-RIAVVGGGVAGMSAATVAASRGHAVTLFEANADVGGQFNFAKVVPGKEEFHETIRYFKVQLQK-TGVDVRLNTRVNREQLEREGFDEVIVATGVVPRALKIEGSNSPQVLSYAQVLQGAEVGRKVAVIGAGGIGFDVSEFLLKPPHQPQSQPLAEWQREWGVDPDPNYVSEGGMQPPVVEPAIREIYLLQRKTTPLGVGLGKTSGWVHRAQLKKHGVRMLRGVQYKAVTDEGLWIEHNGQDQLLRVDTVVVCAGQESVKDLMPKEGESTIANYHIIGGAKLAAELDAKRAIKEGAELAAQL 3K30 Chain:A ((11-489)) ---YDVLFEPVQIGPFTTKNRFYQVPHCNGMG---YRDPSAQASMRKIKAEGGWSAVCTEQVEIHATSDIAPFIELRIWDDQDLPALKRIADAIHEGGGLAGIELAHNGMNAPNQLSRETPLGPGHLPVAPDTIAPIQARAMTKQDIDDLRRWHRNAVRRSIEAGYDIVYVYGAHGYSGVHHFLSKRYNQRTDEYGGSLENRMRLLRELLEDTLDECAGRAAVACRITVEEEIDGGITREDIEGVLRELGEL-PDLWDFAMGSWEG---DSVTSRFAPEGRQEEFVAGLKKLTTKPVVGVGRFTSPDAMVRQIKAGILDLIGAARPSIADPFLPNKIRDGRLNLIRECIGCN-ICVSGDLTMSPIRCTQNPSMGEEWRRGWHPERIRAKESDARVLVVGAGPSGLEAARALGVRGYDVVLAEAGRDLGGRVTQESALPGLSAWGRVKEYREAVLAELPNVEIYRESPMTGDDIVEFGFEHVITATGATWR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3K30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2867 61503 21.45 133.41 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 21.45 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_3K30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K30-query.scw PDB file : Tito_Scwrl_3K30.pdb: