Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITHSLKDKVVLITGGAKNLGGLISRKFAEQGAKLAIHYNSSETQADAEQTLADVKALGADAILIQADLTDIDNIEKLFLDTKQHFGGIDIAINTVGKVLKKPILETTEQEFDSMSDINSKIAYFFIQSAGRHLNNGGKICSIVTSLLAAYT-GFYSTYEGLKAPVEHYTRTASKEFGDRQISVTAVAPGPMDTPFF-------------YGQESAEAVAYHKSASALGGLTKIEDIEPLVRFLVT-DGWWITGQTIFANGGYTTR 4FIZ Chain:A ((16-268)) -----LDGKVALVTGSGRGIGAAVAVHLGRLGAKVVVNYANSTK--DAEKVVSEIKALGSDAIAIKADIRQVPEIVKLFDQAVAHFGHLDIAVSNSGVVSFGHLKDVTEEEFDRVFSLNTRGQFFVAREAYRHLTEGGRIVLTSSNTSKDFSVPKHSLYSGSKGAVDSFVRIFSKDCGDKKITVNAVAPGGTVTDMFHEVSHHYIPNGTSYTAEQRQQMAAHASPLHRNGWP--QDVANVVGFLVSKEGEWVNGKVLTLDGG----

General information: TITO was launched using: RESULT: Template: 4FIZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1295 -60483 -46.71 -254.13 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -46.71 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_4FIZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FIZ-query.scw PDB file : Tito_Scwrl_4FIZ.pdb: