Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQYMTHRCLIAPPEMADDFFANTVIYLARHDEEGAQGIIINRPAGIQIKELLNDLDIDADNVNPHEVLQGGPLRPEAGFVLHTGQPTWHSSIAVGENVCITTSKDILDAIAHNEG-----VGRYQIALGYASWGKNQLEDEIARGDWLICDADMDLIFNLPYDDRWDAAYKKIGVDRTWLASEIGHA 2HRX Chain:A ((20-160)) ---------VLVAAPSMESEDFARSVILIIEHSEYATFGVNLASRSDVAVFNVIPEWVPCV--TKPQALYIGGPLNQQS--VVGVGVTAQGVDAARVDNLTRLANRLVMVNLGADPEEIKPLVSGMRLFAGHAEWAPGQLAQEIENGDWFVAPA-----------------------------------

General information: TITO was launched using: RESULT: Template: 2HRX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 627 -56071 -89.43 -412.29 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -89.43 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.251

(partial model without unconserved sides chains): PDB file : Tito_2HRX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HRX-query.scw PDB file : Tito_Scwrl_2HRX.pdb: