Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALTNEEILNAVAEKTVLELVELISAFEEKFNVSAAAVAVAAPAGGAAAAA----EEQSEFNVELTSFGANKVAVIKAVREATGLGLKEAKDLVE--GAPQ-VLKEGVSKEEGEELKKKLEEAGATVTLK 1DD4 Chain:A ((1-128)) --MTIDEIIEAIEKLTVSELAELVKKLEDKFGVTAAAPVAVAAAPVAGAAAGAAQEEKTEFDVVLKSFGQNKIQVIKVVREITGLGLKEAKDLVEKAGSPDAVIKSGVSKEEAEEIKKKLEEAGAEVELK

General information: TITO was launched using: RESULT: Template: 1DD4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 382 14005 36.66 115.74 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : 36.66 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.615

(partial model without unconserved sides chains): PDB file : Tito_1DD4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DD4-query.scw PDB file : Tito_Scwrl_1DD4.pdb: