Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFTRSQIFLACALASSLLFSACSKENKPQKEDALTATAPATGEASTIIERNAKQRLIETLQKQFKNANINVKILDIKPTEVPNLYWVNLEGMTSVYTTSDGKYLIQGDVIRLGGKELHNIGDSLQASENKKHLAALKNEDLIAYPAKGGKAKHVIYVFTDVSCPYCHKLHEHLAEINEKSIEVRYIAWPRG---EQFMPTMEAVWCSADRKAAFNQAVQGINIPPAQCKNPVREQYQLGLNMGVNGTPAIYNVDGEYLGGYLTPDELIKRLDK 4I5Q Chain:A ((16-214)) -------------------------------------------------------------------AKLGVQSTEIQASPVAGMKTV-LTHSGVLYVTDDGKHIIQGPMYDVSGAHPVNVTNKLLMSQ----LNALEKE-MIVY--KAPDEKHVITVFTDITCGYCHKLHEEMKDYNALGITVRYLAFPRQGLESQAEQDMKSIWCAKDKNKAFDDAMAGKGVKPASCDVNIADHYALGVQLGVSGTPAIVLSNGYVVPGYQGPKEMKAFLDE

General information: TITO was launched using: RESULT: Template: 4I5Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 936 -73245 -78.25 -373.70 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -78.25 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.675

(partial model without unconserved sides chains): PDB file : Tito_4I5Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I5Q-query.scw PDB file : Tito_Scwrl_4I5Q.pdb: