Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQSAGQLFRDAVAQEKPLQVVGTINANHALLAKRAGYKAIYLSGGGVAAGSLGLPDLGISNLDDVLTDVRRITDVCDLPLLVDADTGFGASAFNIARTTKALIKFGAAAMHIEDQVGAKRCGHRPNKAIVTQEEMVDRIKAAVDARGDDSFVIMARTDALAVEGLQAAIDRAGAYIEAGADMLFPEAITELDMYKQFAQKTGVPILANITEFGSTPLFTTEELASADVSLALYPLSAFRAMNKAAETVYETLRKEGTQKNVVDIMQTRKELYERINYYAFEDYLDNAFAKKK 3EOO Chain:A ((8-295)) ----SAGAKFRAAVAAEQPLQVVGAITAYAAKMAEAVGFKAVYLSGGGVAANSLGIPDLGISTMDDVLVDANRITNATNLPLLVDIDTGWG-GAFNIARTIRSFIKAGVGAVHLEDQVGQKRCGHRPGKECVPAGEMVDRIKAAVDARTDETFVIMARTDAAAAEGIDAAIERAIAYVEAGADMIFPEAMKTLDDYRRFKEAVKVPILANLTEFGSTPLFTLDELKGANVDIALYCCGAYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAQLYDYLGYYAYEEKLDQLFNQG-

General information: TITO was launched using: RESULT: Template: 3EOO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1381 -165362 -119.74 -574.17 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -119.74 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_3EOO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EOO-query.scw PDB file : Tito_Scwrl_3EOO.pdb: