Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMNKQPQNSALVVVDVQNGFTPGGNLAVADADTIIPTINQL---AGCFENVVLTQDWHPDNHISFAANHPGKQPFETIELDYGSQVLWPKHCIQGTHDAEFHPDLNIPTAQLIIRKGFHAHIDSYSAFMEADHTTMTGLTGYLKERGIDTVYVVGIATDFCVAWTALDAVKQGFKTLVIEDACKGIDLNGSLEQAWQTMQQQGVIRIQSTDLLNEC 3PL1 Chain:A ((3-185)) ----------ALIIVDVQNDFCEGGSLAVTGGAALARAISDYLAEAADYHHVVATKDFHID-----PGDHFSGTP------DYSSS--WPPHCVSGTPGADFHPSLDTSAIEAVFYKG--AYTGAYSGFEGVDENG-TPLLNWLRQRGVDEVDVVGIATDHCVRQTAEDAVRNGLATRVLVDLTAGVSADTTV-AALEEMRTASVELVCS-------

General information: TITO was launched using: RESULT: Template: 3PL1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 970 -31452 -32.42 -174.73 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -32.42 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_3PL1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PL1-query.scw PDB file : Tito_Scwrl_3PL1.pdb: