Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQFAVIGNPIEQSRSPELHHAFAEKTGVDLNYQKRLAPLDGFESSMRSFFAEGGSGMNVTVPFKEQAFALCNVLTERAQIAKAVNTL-WMENGKLHGDNTDGQGLVAAIQALEWNLENTTILILGAGGATRGVIYPLVQAGAKKIVIANRTLARAEQLVDDLKTAVPQAQLQAISLNDLE-GDFDIVINATSASLSGDALQL-PEKLKFKYA-YEMAY--GKPSSFLDQAKQRNVP-YAEGFGMLVGQAIEAFSIWNGVRPQLKDFL 1P74 Chain:A ((4-258)) ----YAVWGNPIAQSKSPLIQNKLAAQTHQTMEYIAKLGDLDAFEQQLLAFFEEGAKGCNITSPFKERAYQLADEYSQRAKLAEACNTLKKLDDGKLYADNTDGIGLVTDLQRLNWLRPNQHVLILGAGGATKGVLLPLLQA-QQNIVLANRTFSKTKELAERFQ---PYGNIQAVSMDSIPLQTYDLVINATSAG-----ASVDAEILKLGSAFYDMQYAKGTDTPFIALCKSLGLTNVSDGFGMLVAQAAHSFHLWRGVMP------

General information: TITO was launched using: RESULT: Template: 1P74.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1312 -65355 -49.81 -268.95 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -49.81 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_1P74.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P74-query.scw PDB file : Tito_Scwrl_1P74.pdb: