Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIAAQVIKNKSEQAIFTISPEATVLEAIT-IMAEKGIGALVVAEGEQVVGILSERDYTRKVTLMERSSYSTTVAEIMTAKVITVGLNNTVEECLQLMTDRHLRHLPVLDNGKLVGFISIGDLVKAAMEDQKVLIEQLKQYISG 5G5X Chain:A ((8-109)) ---------------VVTVTPEMTVSEVIDLILKTKHLGFPVV-EGERLVGIITLHDII-GVEPEER------VGNIMSREVVAVSPNQSAFEAFKIMSEMGIGRLPVVEHGRVVGIVSRSDLMR-------------------

General information: TITO was launched using: RESULT: Template: 5G5X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 434 -57898 -133.40 -573.24 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -133.40 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_5G5X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G5X-query.scw PDB file : Tito_Scwrl_5G5X.pdb: