Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLQAVYLTPIELHQTPDWSDRPYNIHRDPLWKTEKQGKEFDDVWFYHCDHLGTPQEMTDHTGAIIWKAEYKAWGECKAEK----AKSNFFENSEIISNNIRFQGQYFDEETGLHYNRYRYY-SPYVGRFVSKDPIGLLGGSNNYAYAPSPTEWVDPLGLSCQKIPKGFKSFGQLKQFGQAMQAGFSKLGFKGATMYMQGSAHSGRSFETGKAFDDGRVSDFDIAVVQPELFEKAKKMGIAKGNRTLPIEINSVEANKLGINGVLQKMSKLAGGRDVNVMIFDSPESAKAKAEGTRIPANCM 4TKX Chain:L ((125-231)) ----------------------------------------------------SPEELTNIIDKVLMYEKATMPDKSYLEKVLLIAGADYSWNSQVGQPTIKYGMQYYYNQEHGYTDVYNYLKAPYTGCY-SHLNTGV--SFANYTAHGSETAWADPLLTTSQ-------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TKX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 315 -1311 -4.16 -12.85 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain L : 0.60 3D Compatibility (PKB) : -4.16 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.60 QMean score : -0.060

(partial model without unconserved sides chains): PDB file : Tito_4TKX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TKX-query.scw PDB file : Tito_Scwrl_4TKX.pdb: