Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKRLIGLFSPDLAIDLGTANTLIYAPGRGIILNEPTVVAIRHSGSQKIVAAVGLDAKQMLGRTPANISAIRPMKDGVIADFEVTETMLNQFIGKVHEKRLFPPAPRVVVCVPCKSTLVERRAIREAVFNASARDVRLIEEPMAAAIGAGMPVEQACGSMVVDVGGGTTEIAIISLQGCVYADSLRIGGDVFDEQIINYVRKAHGCVIGETTAEIIKKEVGMAV--SDGTTLEIEVRGRNLAEGVPRAITVTSDEITQAISDPLQSIVSAVKSALEQTPPELSSDIAERGIVLTGGGALLRNLDKLLAQETGLPVVVAEDPLTCVTRGGGKVLEFFDDPNHDMLFVG 4CZK Chain:A ((2-327)) ---------ISNDIAIDLGTANTLIYQKGKGIVLNEPSVVALRN----KVVHAVGIEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKYFIRKVHNRK-GSGNPKVIVCVPSGATAVERRAINDSCLNAGARRVGLIDEPMAAAIGAGLPIHEPTGSMVVDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKEIGTARAP----GLSIDVKGRDLMQGVPREVRISEKQAADALAEPVGQIVEAVKVALEATPPELASDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLEH------------

General information: TITO was launched using: RESULT: Template: 4CZK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1891 -64531 -34.13 -204.21 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -34.13 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_4CZK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZK-query.scw PDB file : Tito_Scwrl_4CZK.pdb: