Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELNPTEKDKLLIFTAGLVAERRKARGLKLNYPEAVAFISAALLEGARDGMTVSELMHFGTTLLKRKDVMDGVPEMIAEVQVEATFPDGSKLVTVHQPIV 1FWC Chain:A ((1-100)) MELTPREKDKLLLFTAALVAERRLARGLKLNYPESVALISAFIMEGARDGKSVASLMEEGRHVLTREQVMEGVPEMIPDIQVEATFPDGSKLVTVHNPII

General information: TITO was launched using: RESULT: Template: 1FWC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 371 -52636 -141.87 -526.36 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -141.87 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_1FWC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FWC-query.scw PDB file : Tito_Scwrl_1FWC.pdb: