Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQPNDYSKIYDIAVIGGGINGVGIANDAVGRGLSVFLCEKDDLASHTSSASSKLIHGGLRYLEYKEFRLVREALAEREVLLAKAPHIIKPMRFIMP-HRPHLRPAWLIRAGLFFYDHLGKREKLLGSNRIYFKEDSPL--KPAITRGFEYSDCTVDDARLVVLNALQAKEKGAKVVTRKRCVK-AYRQQELWYLELQS--GAEFYQVRAKAIVNAAGPWVEEIISENLNLSSPYQIRLIQGSHIVVPK-LYDCHKAFIMQNE-DRRIVFAIPYLEKYTLIGTTDQEYTGDPQKVEITDVEIDYLLTVTNSHFKK-QLTRADIVSQYSGVRALCDDESDNPSAITRDYTLALQAEDKTTPLLSVFGGKITTYRKLAEAALEHLAPFFNDMAEEWTADDPLPG--AENWTTLEDLINQIKTRVS--GISDSLANRWAHAYGTRVWNMLKERNAIEQLGQHFG-HDLFECEVRYLCEYEWAHTAEDILWRRSKLGLAFDEKQVKVLEAYLSERRLKDDAA 3DA1 Chain:A ((36-495)) ----------------------------DAQVRGIQTGLVEMNDFASGTSSRSTKLVHG---------------VGKERAIVYENAPHVTTPEWMLLPIFK------------------------RYMLNEKQTLEKEPLLRKENLKGGGIYVEYRTDDARLTLEIMKEAVARGAVALNYMKVESFIYDQGKVVGVVAKDRLTDTTHTIYAKKVVNAAGPWVDTLREKDRSKHGKY-LKLSKGVHLVVDQSRFPLRQAVYFDTESDGRMIFAIPR-EGKTYIGTTDTFYDKDIASPRMTVEDRDYILAAANYMFPSLRLTADDVESSWAGLRPLIHE-----------DEIFFS----DSGLISIAGGKLTGYRKMAERTVDAVAQGLNVNEPCTTAAIRLSGGLAEGAQGFPRFLDEASRKGAKLGFDADEVRRLAKLYGSNVDHVLNYAYEGKEEAEHYGLPALLLGQLQYGVEQEMVATPLDFFVRRT----------------------------

General information: TITO was launched using: RESULT: Template: 3DA1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2000 56124 28.06 142.81 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 28.06 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_3DA1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DA1-query.scw PDB file : Tito_Scwrl_3DA1.pdb: