Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTATALTLACSLLSVMSISQAQAADNIDVSFQTILQQERNWAGLQSKSLKVGDITWSYSEGGSSTKPTLLLIHGLAGSRDNWNRVAHYLTTNYHVIIPDLPGSGETIVSHDFDYSVPNLAEKLRRFVEAANLKGPIHIAGHSLGGSIALLYAGQYPFETKSLFLVDSGGIFRSANTIYLKDLTYLKQLLVSKKGDFNYLLKQTMFNPPFIPREFLQ--AQEKLMINQAPQTQKLVDQLI-----------ALNKVYTPDSFAVLTKTIDAPTLILWGKQDKIINVEVANELKRLLKNAQPPVILENVGHMPILEAEQLVIQQYVPFLLKVETNQSSKTTTP 4UHE Chain:A ((14-273)) -----------------------------------------------------GEIELAFEDTGTGLP--VLLVHGFPLDRTMWKAQREELCDEFRVIVPDLRGFGESQVIPGV-ATMEAMADDLAGLCNHLGLTGKIVLGGLSMGGYVAFAFARKYRDRLAGLILCDTRARPDSPE---------------AKENRRRVAERVRREGPGFIAEEMIPRLCCESTFRNH-PEVIEKIRQMILSAPPEGVAAAALGMAERPDSTDLL-PALSCPTLVLVGQFDAISPPEEMEAMARTIPQSQ-FVVIPDAGHLPPMEQPERVTQAIREWLRKVHT---------

General information: TITO was launched using: RESULT: Template: 4UHE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1455 -77541 -53.29 -313.93 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -53.29 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_4UHE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UHE-query.scw PDB file : Tito_Scwrl_4UHE.pdb: