Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEMMMKSIKHLAFPLLSAALVMTGCASRKPATTATTGTTNPSTVNTTGLSEDAALNAQNLAGASSKGVTEANKAALAKRIVHFDYDSSDLSTEDYQTLQAHAQFLMANANSKVALTGHTDERGTREYNMALGERRAKAVQNYLITSGVNPQQLEAVSYGKEAPVNPGHDESAWKENRRVEINYEAVPPLLK 2HQS Chain:H ((7-110)) -------------------------------------------------------------------------------IVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYKVTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDEAAYSKNRRAVLVY--------

General information: TITO was launched using: RESULT: Template: 2HQS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 458 -22850 -49.89 -219.71 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain H : 0.68 3D Compatibility (PKB) : -49.89 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_2HQS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HQS-query.scw PDB file : Tito_Scwrl_2HQS.pdb: