Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFPHHTEHHAIQRSGWLRASVLGANDGIISVTSLIMGMAASGASSHTLFITCVAGLISGATSMAAGEYISVKSQEDIEKSDLAIEAKELKKYPQKELDELTQIYISRGLSKELAKEVAIQLTTHDALGAHARDEIGIHENTAANPIQAALSSAASFSFGAFFPMLAILFSPEHLI-------MPSVLITGIAALAILGA-LSSYFAGTSKIKGSLRITLWGILAMAFSSWIGSLFNVTPL 3BHS Chain:A ((147-256)) ------------------------------------------------------------------------------------------------------------------------------AFGAYFGLGVSIALTTAAQRAQPIESDATSDRFSMLGSMVLWLFWPSFATAIVPFEQMPQTIVNTL--LALCGATLATYFLSALFHKGKASIVDMANAALAGGVAIGSVCNI---

General information: TITO was launched using: RESULT: Template: 3BHS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 198 -15311 -77.33 -150.10 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -77.33 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_3BHS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BHS-query.scw PDB file : Tito_Scwrl_3BHS.pdb: