Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPITSENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKNEIEKDMSLIFDLVDYVSY-PQIVTLKPKSVQTVAFHVRMPNE-RFDGVLAGGITFQEIQTEKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNL--TLHEVKPDQINARNVILANVQNDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYHLTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNNWIYILVASLFLLFILLCILLFVLRKKEEKEK 5GHU Chain:A ((2-213)) ------------------------------------DIVISGTR-----------VIYK--SDQK-SVNVRLENKGNNPLLVQSWLDT------GD------------------TVPFTATPPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPP------------LLQLAFRTRIKLFYRPDG--LKGNPSEAPLALKWFWSGS--KASLRVTNPTPYYVS-FS-SGDLEAS--GKRY---PIDVKMIAPFSDEVMKVNGLNGKANSAKVHFYAIND-FG--------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5GHU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 920 5294 5.75 28.31 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 5.75 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.197

(partial model without unconserved sides chains): PDB file : Tito_5GHU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GHU-query.scw PDB file : Tito_Scwrl_5GHU.pdb: