Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIRKALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRI-PSETI--IHTLEETEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIYEYFENELSLTIAY----AQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLED--TRCFEYSESVHRLDKFRFVEFARRRKV 4ZS8 Chain:A ((43-254)) ---------------------PGTPVPPERTLAAEFDTSRTTVRQALQELVVEGRLERIQGKGTFVA-KPKVSQALQ-LTSYTEDMRAQGLEPTSQLLDIGYITADDRLAGLLDITA---GGRVLRIERLRMANGEPMAIETTHLSAKRFPAL-----RRSLVKYTSLYTALAEVYDVHLAEAEETIETSLATPREAGLLGTDVGLPMLMLSRHSQDRTGQPVEWVRSVYRGDRYKFVARLKR---

General information: TITO was launched using: RESULT: Template: 4ZS8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 887 6043 6.81 29.77 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 6.81 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_4ZS8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZS8-query.scw PDB file : Tito_Scwrl_4ZS8.pdb: