Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWANLGFLVTGIGLPFLGVIAIGVSKSS-GVYELAKR-INQTY-AMVFTILLYLVIGPFFALPRLATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGALLLLAFLNPLGSVN-QAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIADHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD 3GIA Chain:A ((3-303)) --LKNKKLSLWEAVSMAVGVMIGASIFSIFGVGAKIAGRNLPETFILSGIYALLVAYSYTKLGAKIVSNAGPIAFIHKAIGDNIITGALSILLWMS--YVISIALFAKGFAGYFLPL-INAPINTFNIAITEIGIVAFFTALNFFGSKAVGRAEFFIVLVKLLILGLFIFAGLIT--IHPSYVIPDLAPSAVSGMIFASAIFFLSY---MGFGVITNASEHIEN-----PK---KNVPRAIFISILIVMFVYVGVAISAIGNLPIDELIKASENALAVAAKPFLGNLGFLLISIGALFSISSAMNATIYGGANVAYSLA---------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GIA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1485 -207569 -139.78 -698.89 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -139.78 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_3GIA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GIA-query.scw PDB file : Tito_Scwrl_3GIA.pdb: