Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDDMLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHFHEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKRAKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLEDPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVDKIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADSGWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFFSFGDAMFVK 2FZP Chain:A ((202-237)) ------------------------------------------------------------------------------------------------WVNSLQPARVTRWGGMISTPDAVLQAVIKRSLVESG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FZP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 19 -2351 -123.74 -65.31 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -123.74 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_2FZP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FZP-query.scw PDB file : Tito_Scwrl_2FZP.pdb: