Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTDILVCLSEKQNGRRNNMSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALVGNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMS----LGISVPV-----ALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMA-ANVPAEGGDAPMISWLAVLLPVLGVVLLD--LP----------IILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDWIP----QSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLF----P--LFAITTIA--PQHLDITQS-WVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA 5ULE Chain:C ((38-444)) -----------------------------------IAVLWLTEALHVTVTAILVPVMAVFFGIFETQ-------------AA--------LNNFANSIIFLFLGGFALAAAMHHQGLDKVIADKVLAMAQGKMSVAVFMLFGVTALL--SMWISN-T-ATAAMMLPLVLGVLSKVDADKQRSTYVFVLLGVAYSASIGGIATLVGTG--PNAIAAAE--V-GLSFTDWMKFGLPTAMMMLPMAIAILYFLLKPTLNGMFELDRAPVNWDKGKVVTLGIFGLTVFL-WIFSSPINAALGGFKSFDTLVAL-GAIL-MLSFARVV--HWKEI--------QKTA---DWGILLLFGGGLCLSNVLKQTGTSVFLANALSDMVSHMGIFVVILVVATFVVF------LTEFTSN--VATTTLLIP-VFATVAEAFGMSPVLLSVLIAVAASCAFMLPVATPPNAIVFASGHIKQSEMMRVGLYLNIACIGLLTAIAMLFW---

General information: TITO was launched using: RESULT: Template: 5ULE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1881 -301461 -160.27 -810.38 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -160.27 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_5ULE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ULE-query.scw PDB file : Tito_Scwrl_5ULE.pdb: