Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPILRKLYKDD----D-EYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA 2DO5 Chain:A ((6-42)) -------------------------------------------AQELQAKLAEIGAPIQGNREELVERLQSYTRQTGIVL-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2DO5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 60 1946 32.43 60.81 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.40 3D Compatibility (PKB) : 32.43 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.40 QMean score : 0.701

(partial model without unconserved sides chains): PDB file : Tito_2DO5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DO5-query.scw PDB file : Tito_Scwrl_2DO5.pdb: