Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIAVEKVSAEEARLMSTGFLFPGQGSQKLGMLADIPEMY------MERLENVTGYRLV-----DKEENYQDTVFIQLALLTKAV-FYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVYHRAVLMKEAYPSGY-AMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSIEGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKNTDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKFGVDKGIRTINLDQHGIDDATFLYQKWR 1MLA Chain:A ((6-290)) --------------------FVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDLWALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCAGVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAE-GQVVSPVNFNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAKITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVGPGKVLTGL--TKRIVD------------------------

General information: TITO was launched using: RESULT: Template: 1MLA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1510 5669 3.75 20.84 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 3.75 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_1MLA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MLA-query.scw PDB file : Tito_Scwrl_1MLA.pdb: