Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKNLPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQ--KWLSSKAAKVELIPLKNNQQAGRYLDLKVSIQYDEWGY--DD--ILNFRLPADVQKQIEGEKIELLDWVNENLEETYVHEAN--GEREEQRKDFSSE 1U7H Chain:A ((241-342)) ------------------------------------------------------------------------------RNARVFVEYEPQTRIEGEIQQLPADFPVVDLWRVLRGETEGRQSDSQVTV-FDSVGFALEDYTVLRYVL-QQAEKRGMGTKIDLVPWVEDDPKDLFSHTRGRAG------------

General information: TITO was launched using: RESULT: Template: 1U7H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 214 9046 42.27 96.23 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 42.27 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_1U7H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U7H-query.scw PDB file : Tito_Scwrl_1U7H.pdb: