Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQEQIEKKKEEKQKAEKAALEKK 2AP3 Chain:A ((3-23)) -MGIQRPTSTTTDKKEI-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2AP3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 9 852 94.61 53.22 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 94.61 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_2AP3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AP3-query.scw PDB file : Tito_Scwrl_2AP3.pdb: