Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSIIAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSFLLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQPLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN 3STA Chain:V ((4-190)) ---IPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSIVSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSFLLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQSIEKIQKDTDRDNFLTAEEAKEYGLIDEVM-------

General information: TITO was launched using: RESULT: Template: 3STA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain V - contact count / total energy / energy per contact / energy per residue : 844 -117787 -139.56 -629.88 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain V : 0.95 3D Compatibility (PKB) : -139.56 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_3STA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3STA-query.scw PDB file : Tito_Scwrl_3STA.pdb: