Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAVNRANEDIITLGANAASKIVTEKDRE--LIDMVIVGTESGIDHSKASAVIIHHLLKIQSFARSFEVKEACYGGTAALHMAKEYVKNHPE--RKVLVIASDIARYGLASGGEVTQGVGAVAMMITQNPRILSIEDD-SVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWN--RHKELSGRGL---------EDYQAIAFHIPYTKMGKKALQSVLDQTD--------------EENQERLMARYEESIRYS----RRIGNLYTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQEMLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI 5HWQ Chain:A ((4-414)) -RVGIEALAVAVPSRYVDIEDLARARGVDPAKYTAGLGAREMAVTDPGEDTVALAATAAARLIRQQDVDPSRIGMLVVGTETGIDHSKPVASHVQGLLKLPRTMRTYDTQHACYGGTAGLMAAVEWIASGAGAGKVAVVVCSDIARYGLNTAGEPTQGGGAVALLVSEQPDLLAMDVGLNGVCSMDVYDFWRPVGRREALVDGHYSITCYLEALSGAYRGWREKALAA-GLVRWSDALPGEQLARIAYHVPFCKMARKA-HTQLRLCDLEDAADAAASTPESREAQAKSAASYDAQVATSLGLNSRIGNVYTASLYLALAGLLQHEAGALAGQRIGLLSYGSGCAAEFYSGTVGEKAAERMAKADLEAVLARRERVSIEEYERLMK--LPADAPEAVAPSPGAFRLTEIRDHRRQY--

General information: TITO was launched using: RESULT: Template: 5HWQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2418 418 0.17 1.11 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 0.17 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_5HWQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HWQ-query.scw PDB file : Tito_Scwrl_5HWQ.pdb: