Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNLKSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE 2NZU Chain:L ((2-88)) -AQKTFTVTADSGIHARPATTLVQAASKFDSDINLEFNGKTVNLKSIMGVMSLGIQKGATITISAEGSDEADALAALEDTMSKEGLGE

General information: TITO was launched using: RESULT: Template: 2NZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 411 -40031 -97.40 -460.12 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain L : 0.93 3D Compatibility (PKB) : -97.40 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_2NZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NZU-query.scw PDB file : Tito_Scwrl_2NZU.pdb: