Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEFTNS----------DSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDINMGCPVPNVAERGKGSGLILRPEVAAQLIEA---AKAGGLPVSVKTRIGYTEMSEMEEWISHLLHQDIANLSVHLRTRKEMSKVD-AHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGEELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSITGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ 4BF9 Chain:A ((26-254)) -------------VLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRIC-PELQNASR-----TPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGDI---RQRL--NIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIP----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4BF9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1195 19455 16.28 90.49 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 16.28 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4BF9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BF9-query.scw PDB file : Tito_Scwrl_4BF9.pdb: