Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQLLTGKVQEITVINAYVDECDFE---QELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEEDFF 2JZO Chain:A ((5-104)) -------------------------IVIGTHGWAAEQLLKTAEMLLGEQENVGWID-FVPGENAETLIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKE-HYEVIAGVNIPMLVETLMA--------------------------------------

General information: TITO was launched using: RESULT: Template: 2JZO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 411 -33322 -81.08 -343.53 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -81.08 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_2JZO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JZO-query.scw PDB file : Tito_Scwrl_2JZO.pdb: