Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCINIGCIPSKSL------IFNGERGI----------DFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFLSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEKLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFHLGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRGAIAVDEYLRTDADNIWAIGDVKGGLQFTY-ISLDDYRIIMDQLKGENKRTTNNRKAVPYSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDLILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK 2EQ7 Chain:A ((2-443)) ----YDLLVIGAGPGGYVAAIRAAQLGMKVGVVEKE-KALGGTCLRVGCIPSKALLETTERIYEAKKGLLGAKVKGVELDLPALMAHKDKVV-QANTQGVEFLFKKNGIARHQGTARFLSERKVLVEETGEEL--EARYILIATGSAPLIPPWAQVDYER-VVTSTEALSFPEVPKRLIVVGGGVIGLELGVVWHRLGAEVIVLEYMDRILPTMDLEVSRAAERVFKKQGLTIRTGVRVTAVVPEAKGARVEL--EGGEV-LEADRVLVAVGRRPYTEGLSLENAGLSTDERGRIPVDEHLRTRVPHIYAIGDVVRGPMLAHKASEEGIAAVEHMVRGFGH---VDYQAIPSVVYTHPEIAAVGYTEEELKAQGIPYKVGKFPYSASGRARAMGETEGFIKVLAHAKTDRILGVHGIGARVGDVLAEAALALFFKASAEDLGRAPHAHPSLSEILKE---

General information: TITO was launched using: RESULT: Template: 2EQ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2509 -18996 -7.57 -44.70 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -7.57 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_2EQ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EQ7-query.scw PDB file : Tito_Scwrl_2EQ7.pdb: