Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEANMKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNSNNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSEGSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISSE-KVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAISTKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPS----NVTSGVIVRSVQSNMPAN-GHLEKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLES 5FHT Chain:A ((12-326)) ----------------------------------------------------------RSQYNFIADVVEKTAPAVVYIEILDRHP----FL------GREVPISNGSGFVVAA---DGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPKADIATLRIQTKEPL-PTLPLGRSADVRQGEFVVAMGSPFAL--QNTITSGIVSSAQR------L--PQTNVEYIQTDAAIDFGNAGGPLVNLDGEVIGVNTMKVT-------AGISFAIPSDRLREFLHR-------QRRYIGVMMLTLSPSI---LAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVR---TQSQLAVQIRRGRETLTLYVTPEVTE-----

General information: TITO was launched using: RESULT: Template: 5FHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1700 -32884 -19.34 -113.39 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -19.34 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_5FHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FHT-query.scw PDB file : Tito_Scwrl_5FHT.pdb: