Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAQALAIRGEPDSYERQENAWLLEHFLKINSLELKFRLEQELTAQQEQDYLAGLERIQNGEPLAYVTGSQPFWTLDLKVTSDTLVPRPDTEVLVETVLNLNLP-KNANIVDLGTGTGAIALALASERPDWFVTATDIYAPTLEVAKENAQTHGLH-HVKFACGVWYEALE--PQQFDLIVSNPPYIDPEDEHMQALATEPRRALVADHHGLADIEIIIAQGKNWLKPQG-WIALEHGYDQGQAVRGIFAEHGFSEIRTIQDYGRNDRVTLASLLIK 1SG9 Chain:B ((32-280)) ----------------SVLEVLLIVSRVLGIRKEDLFLK-DLGVSPTEEKRILELVEKRASGYPLHYILGEKEFMGLSFLVEEGVFVPRPETEELVELALELIRKYGIKTVADIGTGSGAIGVSVAKFS-DAIVFATDVSSKAVEIARKNAERHGVSDRFFVRKGEFLEPFKEKFASIEMILSNPPYVKSSAHLPKDVLFEPPEALFGGEDGLDFYREF----FGRYDTSGKIVLMEIGEDQVEELKKIVSD-----TVFLKDSAGKYRFLLLNRR--

General information: TITO was launched using: RESULT: Template: 1SG9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1192 818 0.69 3.35 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 0.69 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_1SG9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SG9-query.scw PDB file : Tito_Scwrl_1SG9.pdb: