Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTDTALIMALPNESKGLFE-QA--------------------GIEVHYSGIGKINAAFKAFEVIQKTGCKTLINLGTAGSSS--FNRHDLVEIKTFVQRDMDVSPLGFEVGV-----TPLDDHLAAEIHLQT----HFADLPKGICGTGDSFETGQPKV--------ACDVVDMEGYALAKVCHKLGVRLISVKYITDGADDTAHLDWEENLLLGAQKLLALYQNHF 4F1W Chain:A ((17-238)) --MKIGIIGAMEEEVTLLRDKIDNRQTITLGGCEIYTGQLNGTEVALLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLASTLKVGDIVVSDETRYHDADVTAFGYEYGQLPGCPAGFKADDKLIAAAESCIRELNLNAVRGLIVSGDAFINGSVGLAKIRHNFPDAVAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSTLMV----

General information: TITO was launched using: RESULT: Template: 4F1W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 868 -6469 -7.45 -35.54 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -7.45 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4F1W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F1W-query.scw PDB file : Tito_Scwrl_4F1W.pdb: