Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIVKRSFNMTFAIKKIHHVAYRCKDAKETVEWYKKMLNMDFILAFAEDHVPSTKAFDPYMHLFLDAGQGNVLAFFELPTQPEMGRDENTPQWVQHIAFEVED-LNALLAAKKHLEDNGVKVLG-ITNH----GIFHSIYFFDPNGHRLELTYNDAHAEEKIAKITEEMKTEMLEEWSKTKRAPHHTHFLHEEELGA 3EY7 Chain:A ((3-132)) --------EFLMKISHLDHLVLTVADIPTTTNFYEKVLGMKAVSFGAG-------------RIALEFG-HQKINLHQLGNEFEP-KAQNVRVGSADLCFITDTVLS---DAMKHVEDQGVTIMEGPVKRTGAQGAITSFYFRDPDGNLIEVSTYSN-----------------------------------------

General information: TITO was launched using: RESULT: Template: 3EY7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 449 -30991 -69.02 -249.92 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -69.02 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_3EY7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EY7-query.scw PDB file : Tito_Scwrl_3EY7.pdb: