Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGIKTIGVVGVGVIGASWTALFLYKGFKVKVYGPYPIDEELFKKRIQAN---LSDLLALDQQTDSSHHLQDIFLNLELYNNLKDAVIDADFIQENAPERLDLKQNLYQEITSYCPEKTLIASSSSGLKVSDFQKDATHPERIFLGHPFNPPHLLPLVEIVGGKLTDPQILKKASEFYQSLGKHPIVLNKEVKGHVANRLQAALWREAFSLVKEGVCSAEDVDIAITSGPGLRWALFGPYINMELANQKGFKEAIHHLGPPMTEWWNDMQNFQHSEETTELLEEQTKELLTHYKDIDLSQKRDKGLVDILKLKQQLELD 4DYD Chain:A ((1-251)) MTGITNVTVLGTGVLGSQIAFQTAFHGFAVTAYDINTDALDAAKKRFEGLAAVYEKEVAGA--ADG--AAQKALGGIRYSDDLAQAVKDADLVIEAVPESLDLKRDIYTKLGELAPAKTIFATNSSTLLPSDLVGYTGRGDKFLALHFANHVWVNNTAEVMGTTKTDPEVYQQVVEFASAIGMVPIELKKEKAGYVLNSLLVPLLDAAAELLVDGIADPETIDKTWRIGTGAP---KGPFEIFDIVGLTTAYNISSVS-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DYD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1219 -11842 -9.71 -47.75 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -9.71 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_4DYD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DYD-query.scw PDB file : Tito_Scwrl_4DYD.pdb: