Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILIVHAHPEPLSFTTSLKTTAQQTFKKLGHQVVISDLYAMQFNPLASKEDFL-ELNQPDYFNYALEQRNATKQQLLSPDIQAEIDKVKRADLIILNFPLYWTSVPAILKGWIDRVFVSGLFYGGKRFYNHGGMAGKKAMLCLTLGGRTHMFGENSIHGPIEHYLSPIQRGTLAYTGFEVLPPFIAYHVPYISQEARETILEDYVTYLTHLDQLEPLKFPKLEEFDEKLYPL 1QR2 Chain:B ((4-220)) -KVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPI---------------

General information: TITO was launched using: RESULT: Template: 1QR2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1045 -91589 -87.64 -424.02 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -87.64 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_1QR2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QR2-query.scw PDB file : Tito_Scwrl_1QR2.pdb: