Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSCYLITGGAGFIGSTLANHLG-EEHVVIVVDDLSMGKV---ENLDM------TKNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMILELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYCHLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSRDFVYIDDVIQALLLVANEE-AALGKQFNVGTGKATTLLELIHAINEILGVELTLEYK--E----ERSGDIHDSLADITKIQS-IGYQPKYGILSGMTNYLKTEIN 1SB8 Chain:A ((27-348)) PKVWLITGVAGFIGSNLLETLLKLDQKVVGLDNFATGHQRNLDEVRSLVSEKQWSNFKFIQGDIRNLDDCNNAC--AGVDYVLHQAALGSVPRSINDPITSNATNIDGFLNMLIAARDAK--VQSFTYAASSSTYGDHPGLPKVEDTIGKPLSPYAVTKYVNELYADVFSRCYGFSTIGLRYFNVFGRRQDPNGAYAA----VIPKWTSSMI-QGDDVYINGDGETSRDFCYIENTVQANLLAATAGLDARNQVYNIAVGGRTSLNQLFFALRDGLAEN-GVSYHREPVYRDFREGDVRHSLADISKAAKLLGYAPKYDVSAGVALAMPWYI-

General information: TITO was launched using: RESULT: Template: 1SB8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1793 -16927 -9.44 -55.68 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -9.44 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_1SB8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SB8-query.scw PDB file : Tito_Scwrl_1SB8.pdb: