Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNP---LVVGVLKGAVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDIIDSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDYAEAYRNLPYIGVLKPSVYQSN 4RHT Chain:D ((21-197)) ----DIKSVLLTAEQIQARIAELGEQIGNDY-----QDLLLITVLKGAVLFVTDLARAIPVPTQFEFMAVSSYGS----SGVVRILKDLDRDIHGRDVLIVEDVVDSGLTLSWLSRNLTSRNPRSLRVCTLLRKPD---ANVEIAYVGFDIPNDFVVGYGLDYDERYRDLSYIGTLDP------

General information: TITO was launched using: RESULT: Template: 4RHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 757 -29976 -39.60 -188.53 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.85 3D Compatibility (PKB) : -39.60 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_4RHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RHT-query.scw PDB file : Tito_Scwrl_4RHT.pdb: