Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGIIGAMEEEVKILRENLSEPLSWER---AGALFISGSLGNHEVIVVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADATGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKEILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRSAEMVIEFVKHLV 4YO8 Chain:B ((18-245)) -KIGILGAMREEITPILELFG--VDFEEIPLGGNVFHKGVYHNKEIIVAYSKIGKVHSTLTTTSMILAFGVQKVLFSGVAGSLVKDLKINDLLVATQLVQHDVDLSAFDHPLGFIPESAIFIETSGSLNALAKKIANEQHIALKEGVIASGDQFVHSKER-KEFLVSEFKASAVEMEGASVAFVCQKFGVPCCVLRSISDNADEKAGMSFDEFLEKSAHTSAKFLKSMVDEL-

General information: TITO was launched using: RESULT: Template: 4YO8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1351 -13795 -10.21 -61.31 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -10.21 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_4YO8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YO8-query.scw PDB file : Tito_Scwrl_4YO8.pdb: