Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------MANRGNVHQLLKSKEEIE--YLVTLHTHRY--ANMPLPKQLEKKFWCLN----------LDRTRQNISKFLASGVLT--------YLIFILLALPTDYLVIGVPYITLDFIHCLLSAMNIALALLLFWVFAKFTKLSEHFYLAACGIVF----LTIIINAMLLLSVGNMALKNQSMLLLSFLY------MLGFILSGIK---PLHMLCVGLLAAFLVFAFLILLDVNCDYIALGRALFGSCILGFSISSMLISRERSLFLNNQLAEINEQILRIEASELLHLSQQDALTQISNRRTFDEMFDFFYYRANQEKRPLAVLFIDIDFFKNYSDFTGTRWEIKSSLA--LPQQLKTQFVM---------STLLRVMGA--RSLLYYYLK---HLHRVLIQWLQISIKPLSG-------KRFLMQLL---------- 4XA2 Chain:A ((1-490)) MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAG-KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSAVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYL------LTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIP--QMSAFWYAVRTAVINAASGRQTVDAALAAAQTNAA---AAYQNYIAKSQVSTGLADITAGKTNAETKLAEGLTAALTDVEALGLQKSTNACSTITTSIGTNGASNITCTLKGTSQINSKKIEWIRDADNATNGTTGAWRCKTDVAENLRPKSCGASGS

General information: TITO was launched using: RESULT: Template: 4XA2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1985 -43229 -21.78 -122.81 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -21.78 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_4XA2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XA2-query.scw PDB file : Tito_Scwrl_4XA2.pdb: