Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAQALAIRGEPDSYERQENAWLLEHFLKINSLELKFRLEQELTAQQEQDYLAGLERIQNGEPLAYVTGSQPFWTLDLKVTSDTLVPRPDTEVLVETVLNLNLP-KNANIVDLGTGTGAIALALASERPDWFVTATDIYAPTLEVAKENAQTHGLH-HVKFACGVWYEALE--PQQFDLIVSNPPYIDPEDEHMQALATEPRRALVADHHGLADIEIIIAQGKNWLKPQG-WIALEHGYDQGQAVRGIFAEHGFSEIRTIQDYGRNDRVTLASLLIK 1NV8 Chain:A ((34-282)) ----------------SVLEVLLIVSRVLGIRKEDLFLK-DLGVSPTEEKRILELVEKRASGYPLHYILGEKEFMGLSFLVEEGVFVPRPETEELVELALELIRKYGIKTVADIGTGSGAIGVSVAKFS-DAIVFATDVSSKAVEIARKNAERHGVSDRFFVRKGEFLEPFKEKFASIEMILSNPPYVKSSAHLPKDVLFEPPEALFGGEDGLDFYRE----FFGRYDTSGKIVLMEIGEDQVEELKKIVS-----DTVFLKDSAGKYRFLLLNRR--

General information: TITO was launched using: RESULT: Template: 1NV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1155 -7295 -6.32 -29.90 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -6.32 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_1NV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NV8-query.scw PDB file : Tito_Scwrl_1NV8.pdb: