Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQMKNITD-QEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH 1MQ7 Chain:A ((16-113)) -----------------LPSRAHDGDAGVDLYSAEDVELAPGRRALVRTGVAVAVPFGMVGLVHPRSGLATRVGLSIVNSPGTIDAGY------RGEIKVALINLDPAAPIVVHRGDRIAQ---------------------------

General information: TITO was launched using: RESULT: Template: 1MQ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 409 -40939 -100.09 -422.05 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -100.09 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_1MQ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MQ7-query.scw PDB file : Tito_Scwrl_1MQ7.pdb: