Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKTVKDIDLKDKKVLVRVDFNVPLKD-GVITDDTRIKAALPTINYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEEAIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHASNVGIASTGIP---TVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKADKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAG---DKLVLPIDNVCAPEFSNDVETQVIE-GDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTIGVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAAINDK 3Q3V Chain:B ((7-399)) --IISIKDIDLAKKKVFIRCDFNVPQDDFLNITDDRRIRSAIPTIRYCLDNGCSVILASHLGRPKEIS-S-KYSLEPVAKRLARLLDKEIVMAKDVIGEDAKTKAMNLKAGEILLLENLRFE----KGETKNDENLAKELASMVQVYINDAFGVCHRAHSSVEAITKF-FDEKHKGAGFLLQKEIDFASNLIKHPARPFVAVVGGSKVSGKLQALTNLLPKVDKLIIGGGMAFTFLKALGYDIGNSLLEEELLEEANKILTKGKNLGVKIYLPVDVVAAPACSQDVPMKFVPAQEIPNGWMGLDIGPASVRLFKEVISDAQTIWWNGPMGVFEIDKFSKGSIKMSHYISE-GHATSVVGGGDTADVVARAGDADEMTFISTGG-ASLELIEGKELPGVKALRS-

General information: TITO was launched using: RESULT: Template: 3Q3V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2310 -35531 -15.38 -92.53 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -15.38 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.666

(partial model without unconserved sides chains): PDB file : Tito_3Q3V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q3V-query.scw PDB file : Tito_Scwrl_3Q3V.pdb: