Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPALFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYFHETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVSNMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPENIAEYMAKENVDGALVGGASLQADSFLSLLDAVK 2I9E Chain:C ((3-245)) -RKFVVGGNWKMNGDKKQINEIIGFLKSG-PLNQDTEVVVGVPAIYL-ELVRTCVPASIGVAAQNCYKVPKGAFTGEISPAMIKDVGADWVILGHSERRQIFGESDELIAEKVCHALESGLKVIACIGETLEEREAGKTEEVVFRQTKAIAAKVN--DWSNVVIAYEPVWAIGTGKTATPQQAQDVHKALRQWICENIDAKVGNSIRIQYGGSVTAANCKELASQPDIDGFLVGGASLKPE-FVDIINA--

General information: TITO was launched using: RESULT: Template: 2I9E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1353 -141814 -104.81 -583.59 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.83 3D Compatibility (PKB) : -104.81 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_2I9E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I9E-query.scw PDB file : Tito_Scwrl_2I9E.pdb: